Measurement

Part:BBa_I2055:Design

Designed by: Jason Kelly   Group: iGEM06_MIT   (2007-06-21)


RBS tester with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704


Design Notes

In order to ensure that the spacing between the RBS and the ORF of the gene was optimal, the PstI restriction site had to be omitted from the standard BioBrick site.


Source

This part was made from the combination of parts R0040 and I13401, which were already in the Registry.

References